anti rabbit 53bp1 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti rabbit 53bp1
Anti Rabbit 53bp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+53bp1/pmc12993239-104-21-23
Average 86 stars, based on 1 article reviews
Anti Rabbit 53bp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+53bp1/pmc12993239-104-21-23
Average 86 stars, based on 1 article reviews
anti rabbit 53bp1 - by Bioz Stars,
2026-09
86/100 stars
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Incubation:Article Title: FOXM1 enhances DNA repair in aged cells to maintain the peripheral heterochromatin barrier to senescence enhancers Article Snippet: Blocking was done with 10% FBS (Gibco) diluted in PBS-T for 1 hr at RT. .. Cells were incubated either 1h at RT or overnight at 4°C with primary antibodies diluted in PBS-T + 5% FBS as follows: rabbit anti-H3K9me2, 1:800 (39041, Active Motif); mouse anti-Lamin A/C, 1:100 (4777S, Cell Signaling); mouse anti-p21, 1:400 (clone F-5, sc-6246, Santa Cruz Biotechnology); rabbit anti-p53, 1:400 (clone FL-393, sc-6243, Santa Cruz Biotechnology); mouse anti-γH2AX, 1:1000 (clone JWB301, 05-636, Merck); Article Title: Feedback regulation between FOXM1 and APC/C Cdh1 determines the changes in cell cycle dynamics during aging Article Snippet: .. Cells were incubated overnight at 4°C with primary antibodies diluted in PBS-T + 5% FBS as follows: mouse anti-FZR1 (#SC-56312, Santa Cruz Biotechnology, CA, USA) 1:75, Blocking Assay:Article Title: Genome rearrangements induced by the stimulation of end-joining of DNA double strand breaks through multiple phosphorylation of MRE11 by the kinase PKB/AKT1. Article Snippet: For immunofluorescence analysis of pATM (Ser1981) and pChk2 (Thr68), soluble proteins were extracted before fixation by incubating coverslips with extraction buffer [50 mM Tris–HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, and protease inhibitor cocktail (cOmplete D ow nloaded from https://academ ic.oup.com /nar/article/53/11/gkaf468/8158035 by guest on 10 June 2025 Mini Protease Inhibitor, Roche, 5892970001] for 5 min on ice. .. After blocking in PBS containing 3% BSA and 0.05% Tween 20, immunostaining was performed using the following primary antibodies: mouse anti-53BP1 (1:200, Becton Dickinson, 612522), Article Title: Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence Article Snippet: .. After blocking with 5% skimmed milk solution in 1% Tris-Buffered saline with Tween 20 (TBST) buffer, the membranes were immunoblotted with mouse anti-Flag antibody (Sigma, #F3165, 1:1000), rabbit anti-TDP-43 (Proteintech, #10,782–2-AP, 1:1000), mouse anti-phospho-Histone H2AX (S139) (EMD Millipore, #16–193, 1:1000), rabbit anti-H2AX (Cell Signaling Tech, #2595, 1:1000), rabbit anti-phospho-ATM (S1981) (Abcam, #ab81292, 1:800), rabbit anti-ATM (Abcam, #ab32420, 1:1000), rabbit anti-phospho-53BP1 (S1778) (Cell Signaling Tech, #2675, 1:1000), Article Title: Genome rearrangements induced by the stimulation of end-joining of DNA double strand breaks through multiple phosphorylation of MRE11 by the kinase PKB/AKT1 Article Snippet: For immunofluorescence analysis of pATM (Ser1981) and pChk2 (Thr68), soluble proteins were extracted before fixation by incubating coverslips with extraction buffer [50 mM Tris–HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, and protease inhibitor cocktail (cOmplete Mini Protease Inhibitor, Roche, 5892970001] for 5 min on ice. .. After blocking in PBS containing 3% BSA and 0.05% Tween 20, immunostaining was performed using the following primary antibodies: mouse anti-53BP1 (1:200, Becton Dickinson, 612522), Article Title: Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence. Article Snippet: .. After blocking with 5% skimmed milk solution in 1% Tris-Buffered saline with Tween 20 (TBST) buffer, the membranes were immunoblotted with mouse anti-Flag antibody (Sigma, #F3165, 1:1000), rabbit anti-TDP-43 (Proteintech, #10,782–2-AP, 1:1000), mouse anti-phospho-Histone H2AX (S139) (EMD Millipore, #16–193, 1:1000), rabbit anti-H2AX (Cell Signaling Tech, #2595, 1:1000), rabbit anti-phospho-ATM (S1981) (Abcam, #ab81292, 1:800), rabbit anti-ATM (Abcam, #ab32420, 1:1000), rabbit anti-phospho-53BP1 (S1778) (Cell Signaling Tech, #2675, 1:1000), Immunostaining:Article Title: Genome rearrangements induced by the stimulation of end-joining of DNA double strand breaks through multiple phosphorylation of MRE11 by the kinase PKB/AKT1. Article Snippet: For immunofluorescence analysis of pATM (Ser1981) and pChk2 (Thr68), soluble proteins were extracted before fixation by incubating coverslips with extraction buffer [50 mM Tris–HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, and protease inhibitor cocktail (cOmplete D ow nloaded from https://academ ic.oup.com /nar/article/53/11/gkaf468/8158035 by guest on 10 June 2025 Mini Protease Inhibitor, Roche, 5892970001] for 5 min on ice. .. After blocking in PBS containing 3% BSA and 0.05% Tween 20, immunostaining was performed using the following primary antibodies: mouse anti-53BP1 (1:200, Becton Dickinson, 612522), Article Title: Genome rearrangements induced by the stimulation of end-joining of DNA double strand breaks through multiple phosphorylation of MRE11 by the kinase PKB/AKT1 Article Snippet: For immunofluorescence analysis of pATM (Ser1981) and pChk2 (Thr68), soluble proteins were extracted before fixation by incubating coverslips with extraction buffer [50 mM Tris–HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, and protease inhibitor cocktail (cOmplete Mini Protease Inhibitor, Roche, 5892970001] for 5 min on ice. .. After blocking in PBS containing 3% BSA and 0.05% Tween 20, immunostaining was performed using the following primary antibodies: mouse anti-53BP1 (1:200, Becton Dickinson, 612522), Saline:Article Title: Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence Article Snippet: .. After blocking with 5% skimmed milk solution in 1% Tris-Buffered saline with Tween 20 (TBST) buffer, the membranes were immunoblotted with mouse anti-Flag antibody (Sigma, #F3165, 1:1000), rabbit anti-TDP-43 (Proteintech, #10,782–2-AP, 1:1000), mouse anti-phospho-Histone H2AX (S139) (EMD Millipore, #16–193, 1:1000), rabbit anti-H2AX (Cell Signaling Tech, #2595, 1:1000), rabbit anti-phospho-ATM (S1981) (Abcam, #ab81292, 1:800), rabbit anti-ATM (Abcam, #ab32420, 1:1000), rabbit anti-phospho-53BP1 (S1778) (Cell Signaling Tech, #2675, 1:1000), Article Title: Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence. Article Snippet: .. After blocking with 5% skimmed milk solution in 1% Tris-Buffered saline with Tween 20 (TBST) buffer, the membranes were immunoblotted with mouse anti-Flag antibody (Sigma, #F3165, 1:1000), rabbit anti-TDP-43 (Proteintech, #10,782–2-AP, 1:1000), mouse anti-phospho-Histone H2AX (S139) (EMD Millipore, #16–193, 1:1000), rabbit anti-H2AX (Cell Signaling Tech, #2595, 1:1000), rabbit anti-phospho-ATM (S1981) (Abcam, #ab81292, 1:800), rabbit anti-ATM (Abcam, #ab32420, 1:1000), rabbit anti-phospho-53BP1 (S1778) (Cell Signaling Tech, #2675, 1:1000), |
